HIF-1 Alpha DNA Binding Activity Assay test kit

NameHIF-1 Alpha DNA Binding Activity Assay test kit
Price€ 674.00
Size96 assays
Catalog numberCBA-282
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We send this reagant onRoom temperature
The temperatureerature for storage isUpon receipt, store the Biotinylated Double Stranded DNA and Anti-HIF-1 Alpha Antibody at -20ºC. Store all other components at 4ºC.
Technical file kit.pdf
SDS or safty data sheet (CBA-282) GHS.pdf
Type of reagentCell Based Assays
Related techniquesCell Based Assays, Cell Health Assays>Hypoxia Assays
Use of the reagentCell Biolabs’ HIF-1 Alpha DNA Binding Activity Assay test kit is an immunoassay developed for rapid detection of activated HIF-1 in any protein sample. Active HIF-1 complex is captured on a double stranded oligonucleotide containing an HRE that is attached to the well. HIF-1 Alpha is then detected with an anti-HIF-1 Alpha antibody followed by an HRP conjugated secondary antibody. Each test kit provides sufficient reagents to perform up to a total of 96 assays, and can detect HIF-1 Alpha from human, mouse, or rat. The HIF-1 DNA Binding Activity Assay takes only 4.5 hours to complete compared to a standard 3 day electromobility shift (EMSA) method.
Conjugation and formhost specific
Species compatible tohuman cells and cell lines and other cells
Use of this test kit or productMolecular biology
Antigenthe sequence of the protein
BufferThe concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,53
Molecular weightkiloDalton
This reagent is purified0.98
DescriptionThe HIF-1 Alpha DNA Binding Activity Assay test kit is a α- or alpha protein sometimes glycoprotein present in blood.
TestCellular biology labs supplies other types of Assays as 1.