HIF-1 Alpha DNA Binding Activity Assay test kit

Name HIF-1 Alpha DNA Binding Activity Assay test kit
Price € 674.00
Size 96 assays
Catalog number CBA-282
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We send this reagant on Room temperature
The temperatureerature for storage is Upon receipt, store the Biotinylated Double Stranded DNA and Anti-HIF-1 Alpha Antibody at -20ºC. Store all other components at 4ºC.
Technical file kit.pdf
SDS or safty data sheet (CBA-282) GHS.pdf
Type of reagent Cell Based Assays
Related techniques Cell Based Assays, Cell Health Assays >Hypoxia Assays
Use of the reagent Cell Biolabs’ HIF-1 Alpha DNA Binding Activity Assay test kit is an immunoassay developed for rapid detection of activated HIF-1 in any protein sample. Active HIF-1 complex is captured on a double stranded oligonucleotide containing an HRE that is attached to the well. HIF-1 Alpha is then detected with an anti-HIF-1 Alpha antibody followed by an HRP conjugated secondary antibody. Each test kit provides sufficient reagents to perform up to a total of 96 assays, and can detect HIF-1 Alpha from human, mouse, or rat. The HIF-1 DNA Binding Activity Assay takes only 4.5 hours to complete compared to a standard 3 day electromobility shift (EMSA) method.
Conjugation and form host specific
Species compatible to human cells and cell lines and other cells
Use of this test kit or product Molecular biology
Antigen the sequence of the protein
Buffer The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,53
Molecular weight kiloDalton
This reagent is purified 0.98
Description The HIF-1 Alpha DNA Binding Activity Assay test kit is a α- or alpha protein sometimes glycoprotein present in blood.
Test Cellular biology labs supplies other types of Assays as 1.