| We send this reagant on |
Room temperature |
| The temperatureerature for storage is |
Upon receipt, store the Biotinylated Double Stranded DNA and Anti-HIF-1 Alpha Antibody at -20ºC. Store all other components at 4ºC. |
| Technical file |
kit.pdf |
| SDS or safty data sheet |
(CBA-282) GHS.pdf |
| Type of reagent |
Cell Based Assays |
| Related techniques |
Cell Based Assays, Cell Health Assays >Hypoxia Assays |
| Use of the reagent |
Cell Biolabs’ HIF-1 Alpha DNA Binding Activity Assay test kit is an immunoassay developed for rapid detection of activated HIF-1 in any protein sample. Active HIF-1 complex is captured on a double stranded oligonucleotide containing an HRE that is attached to the well. HIF-1 Alpha is then detected with an anti-HIF-1 Alpha antibody followed by an HRP conjugated secondary antibody. Each test kit provides sufficient reagents to perform up to a total of 96 assays, and can detect HIF-1 Alpha from human, mouse, or rat. The HIF-1 DNA Binding Activity Assay takes only 4.5 hours to complete compared to a standard 3 day electromobility shift (EMSA) method. |
| Conjugation and form |
host specific |
| Species compatible to |
human cells and cell lines and other cells |
| Use of this test kit or product |
Molecular biology |
| Antigen |
the sequence of the protein |
| Buffer |
The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,53 |
| Molecular weight |
kiloDalton |
| This reagent is purified |
0.98 |
| Description |
The HIF-1 Alpha DNA Binding Activity Assay test kit is a α- or alpha protein sometimes glycoprotein present in blood. |
| Test |
Cellular biology labs supplies other types of Assays as 1. |