ApoBrdU-IHC DNA Fragmentation Assay Kit

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Name	ApoBrdU-IHC DNA Fragmentation Assay Kit
Price	€ 702.00
Size	50 assays
Catalog number	K403-50
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Kit's description	Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells. BioVision’s Apo-BrdU-IHCTM Kit is a two-color TUNEL (Terminal deoxynucleotide transferase dUTP Nick End Labeling) assay for labeling DNA breaks to detect apoptotic cells by immunohistochemistry. The kit contains positive/negative control slides for assessing reagent performance; reaction and blocking buffers for processing individual steps in the assay; proteinase K; terminal deoxynucleotidyl tranferase enzyme (TdT), bromodeoxyuridine triphosphate (Br-dUTP), biotin labeled antiBrdU antibody for labeling DNA breaks, horseradish peroxidase streptavidin conjugate, DAB, H₂O₂/Urea tablets for color generation and methyl green solution for counter staining the cells.
Highlights	• Species reactivity: Mammalian • Applications: The TUNEL:based immunohistochemical staining kit provides complete reagents including positive and negative control slides for convenient detection of DNA fragmentation
Kit's benefits	• Simple procedure; convenient • The assay is sensitive and stable • The assay offers an one-step labeling of apoptotic cells.
Category	TUNEL Assay
Kit's other name	none
Contents	• Control Slides-pos/neg • Blocking Buffer • H₂O₂/Urea Tablets • Protease K • DAB Tablets • TdT Enzymes • Br-dUTP • 200X Conjugate • 5X Reaction Buffer • Anti-BrdU-Biotin Antibody • Methyl Green
Storage condition	-20°C
Shipping condition	gel pack
Maximum time can be stored	12 months
Species reactivity	see datasheet
Applications	Inquire
Samples tested	Tissue sections and fixed cell preparations
Test	Biovision supplies other types of Assays as 1.