ApoBrdU-IHC DNA Fragmentation Assay Kit

Name ApoBrdU-IHC DNA Fragmentation Assay Kit
Price € 702.00
Size 50 assays
Catalog number K403-50
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Kit's description Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells. BioVision’s Apo-BrdU-IHCTM Kit is a two-color TUNEL (Terminal deoxynucleotide transferase dUTP Nick End Labeling) assay for labeling DNA breaks to detect apoptotic cells by immunohistochemistry. The kit contains positive/negative control slides for assessing reagent performance; reaction and blocking buffers for processing individual steps in the assay; proteinase K; terminal deoxynucleotidyl tranferase enzyme (TdT), bromodeoxyuridine triphosphate (Br-dUTP), biotin labeled antiBrdU antibody for labeling DNA breaks, horseradish peroxidase streptavidin conjugate, DAB, H₂O₂/Urea tablets for color generation and methyl green solution for counter staining the cells.
Highlights • Species reactivity: Mammalian • Applications: The TUNEL:based immunohistochemical staining kit provides complete reagents including positive and negative control slides for convenient detection of DNA fragmentation
Kit's benefits • Simple procedure; convenient • The assay is sensitive and stable • The assay offers an one-step labeling of apoptotic cells.
Category TUNEL Assay
Kit's other name none
Contents • Control Slides-pos/neg • Blocking Buffer • H₂O₂/Urea Tablets • Protease K • DAB Tablets • TdT Enzymes • Br-dUTP • 200X Conjugate • 5X Reaction Buffer • Anti-BrdU-Biotin Antibody • Methyl Green
Storage condition -20°C
Shipping condition gel pack
Maximum time can be stored 12 months
Species reactivity see datasheet
Applications Inquire
Samples tested Tissue sections and fixed cell preparations
Test Biovision supplies other types of Assays as 1.