ApoDIRECT DNA Fragmentation Assay Kit

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Name	ApoDIRECT DNA Fragmentation Assay Kit
Price	€ 702.00
Size	50 assays
Catalog number	K402-50
	Buy online

Kit's description	Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells. BioVision’s ApoDIRECT In Situ DNA Fragmentation Assay Kit provides complete components including positive and negative control cells for conveniently detecting DNA fragmentation by fluorescence microscopy or flow cytometry. The TUNEL-based detection kit utilizes terminal deoxynucleotidyl transferase (TdT) to catalyze incorporation of fluorescein-12-dUTP at the free 3’-hydroxyl ends of the fragmented DNA. The fluorescein-labeled DNA can then be observed by fluorescence microscopy or analyzed by flow cytometry.
Highlights	• Detection method: Flow cytometry (Ex/Em = 488/520 nm for FITC, and 488/623 nm for PI) and fluorescence microscopy (FITC and rhodamine filters) • Species reactivity: Mammalian • Applications: The TUNEL:based assay kit provides complete components including positive and negative control cells for convenient detection of DNA fragmentation in cultured cells and tissue sections.
Kit's benefits	• Simple procedure; takes ~ 1 hour • Fast and convenient • The assay is sensitive and stable • The assay offers an one-step labeling of apoptotic cells.
Category	TUNEL Assay
Kit's other name	none
Contents	• Positive Control Cells • Negative Control Cells • Wash Buffer • Reaction Buffer • TdT Enzymes • FITC-dUTP • Rinse Buffer • PI/RNase Staining Buffer
Storage condition	-20°C
Shipping condition	gel pack
Maximum time can be stored	12 months
Species reactivity	see datasheet
Applications	Inquire
Samples tested	Cell cultures (adherent and suspension)
Test	Biovision supplies other types of Assays as 1.