ApoDIRECT DNA Fragmentation Assay Kit

Name ApoDIRECT DNA Fragmentation Assay Kit
Price € 702.00
Size 50 assays
Catalog number K402-50
  Buy online
Kit's description Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells. BioVision’s ApoDIRECT In Situ DNA Fragmentation Assay Kit provides complete components including positive and negative control cells for conveniently detecting DNA fragmentation by fluorescence microscopy or flow cytometry. The TUNEL-based detection kit utilizes terminal deoxynucleotidyl transferase (TdT) to catalyze incorporation of fluorescein-12-dUTP at the free 3’-hydroxyl ends of the fragmented DNA. The fluorescein-labeled DNA can then be observed by fluorescence microscopy or analyzed by flow cytometry.
Highlights • Detection method: Flow cytometry (Ex/Em = 488/520 nm for FITC, and 488/623 nm for PI) and fluorescence microscopy (FITC and rhodamine filters) • Species reactivity: Mammalian • Applications: The TUNEL:based assay kit provides complete components including positive and negative control cells for convenient detection of DNA fragmentation in cultured cells and tissue sections.
Kit's benefits • Simple procedure; takes ~ 1 hour • Fast and convenient • The assay is sensitive and stable • The assay offers an one-step labeling of apoptotic cells.
Category TUNEL Assay
Kit's other name none
Contents • Positive Control Cells • Negative Control Cells • Wash Buffer • Reaction Buffer • TdT Enzymes • FITC-dUTP • Rinse Buffer • PI/RNase Staining Buffer
Storage condition -20°C
Shipping condition gel pack
Maximum time can be stored 12 months
Species reactivity see datasheet
Applications Inquire
Samples tested Cell cultures (adherent and suspension)
Test Biovision supplies other types of Assays as 1.