ApoBrdU DNA Fragmentation Assay Kit

Name ApoBrdU DNA Fragmentation Assay Kit
Price € 702.00
Size 60 assays
Catalog number K401-60
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Kit's description Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells. BioVision’s Apo-BrdU In Situ DNA Fragmentation Assay Kit provides complete components including positive and negative control cells for conveniently detecting DNA fragmentation by fluorescence microscopy or flow cytometry. The kit utilizes Br-dUTP (bromolated deoxyuridine triphosphate nucleotides) which is more readily incorporated into DNA strand breaks than other larger ligands (e.g., fluorescein, biotin or digoxigenin). The greater incorporation gives rise to brighter signal when the Br-dUTP sites are identified by a fluorescein labeled anti-BrdU monoclonal antibody.
Highlights • Detection method: Flow cytometry (Ex/Em = 488/520 nm for FITC, and 488/623 nm for PI) and fluorescence microscopy (FITC and rhodamine filters) • Species reactivity: Mammalian • Applications: The TUNEL:based assay kit provides complete components including positive and negative control cells for convenient detection of DNA fragmentation in cultured cells and tissue sections.
Kit's benefits • Simple procedure; takes ~ 1 hour • Fast and convenient • The assay is sensitive and stable • The incorporation of BrdU in the procedure provides greater sensitivity than biotinylated or digoxigenylated methods.
Category TUNEL Assay
Kit's other name none
Contents • Positive Control Cells • Negative Control Cells • Wash Buffer • Reaction Buffer • TdT Enzymes • Br-dUTP • Rinse Buffer • Anti-BrdU-FITC Antibody • PI/RNase Staining Buffer
Storage condition -20°C
Shipping condition gel pack
Maximum time can be stored 12 months
Species reactivity see datasheet
Applications Inquire
Samples tested Cell cultures (adherent and suspension), parafin embedded tissue sections
Test Biovision supplies other types of Assays as 1.