| Shipping Temp |
Room Temp |
| Storage Temp |
Upon receipt, store the Biotinylated Double Stranded DNA and Anti-HIF-1 Alpha Antibody at -20ºC. Store all other components at 4ºC. |
| Detection Method |
Colorimetric |
| Category (Product Type) |
Kit |
| URL link to SDS |
(CBA-282) GHS.pdf |
| UNSPSC |
41116158 |
| Product Line |
Cell Based Assays |
| Short Description |
Cell Biolabs’ HIF-1 Alpha DNA Binding Activity Assay Kit is an immunoassay developed for rapid detection of activated HIF-1 in any protein sample. Active HIF-1 complex is captured on a double stranded oligonucleotide containing an HRE that is attached to the well. HIF-1 Alpha is then detected with an anti-HIF-1 Alpha antibody followed by an HRP conjugated secondary antibody. Each kit provides sufficient reagents to perform up to a total of 96 assays, and can detect HIF-1 Alpha from human, mouse, or rat. The HIF-1 DNA Binding Activity Assay takes only 4.5 hours to complete compared to a standard 3 day electromobility shift (EMSA) method. |
| Description |
The HIF-1 Alpha DNA Binding Activity Assay Kit is a α- or alpha protein sometimes glycoprotein present in blood. |
| Test |
Cell Biolabs supplies other types of Assays as 1. |