||96-well Topoisomerase DNA Cleavage Assay Kit (enzyme not included) includes 300 µl of 10 x reaction buffer, 30 µl of 10 x DNA, 260 µl of SDS solution, 30 µl of 100 mM ATP, 260 µl of proteinase K solutions, 10 ml of TDC matrix, 6 ml of rinse buffer, 500 µl of 10 x dye, a black receiver plate, a V-bottom reaction plate and a TDC filter plate. Topoisomerase is not included in the kit. A plate filtration device (see Note below) and a vacuum line are needed for the plate filtration process.
||Reagents for fluorescence-based 96-well assays of topoisomerase DNA cleavage activity, 96 assays, enzyme is not included
||Bacterial DNA topoisomerases and assays
|Shipped to you
||This product is shipped to you frozen
||Product made-to-order. Delivery is 1-2 weeks upon order confirmation. Please contact us to check the current inventory stock. For larger or bulk quantity a discount may apply.
||For Research Use Only. Produced in USA
||ProF supplies other types of Assays as 1.
||Enzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.